Figure 6

QRT-PCR analysis of SlIPT3 and SlIPT4 expression. Spatio-temporal expression profile during tomato plant development in vegetative organs (A) and reproductive organs (B). Regulation of SlIPT3 and SlIPT4 expression by CK treatment: Effects of tZ (10 μM) treatment on SlIPT3 and SlIPT4 expression (C) and the expression levels of CK response regulators SlARR1, SlARR4 and SlARR12 (D). Actin and GAPDH were used as internal controls for normalization of SlIPT3 and SlIPT4 transcript levels. Data represent means and SD of three replicates. *statistically significant difference from time 0 h (unpaired two-tailed Student’s t-test, p ≤ 0.05). **statistically significant difference from time 0 h after Šidák correction for testing of multiple points (multiple unpaired two-tailed Student’s t-test, overall α = 0.05, individual p ≤ 0.0127). YL, young leaves; OL, old leaves; Ro, roots; St, stem; Bud, bud; Flo, flower; Gre, green stage; Bre, breaker stage; Red, red stage of tomato development.