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Table 1 Identification of differentially expressed proteins in the roots of Rangpur lime and ‘Sunki Maravilha’ mandarin subjected to water deficit

From: Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit

ID spot a

Identified protein reference organism b

Accession number c

Mascot score/ P -value d

Mr Theor/Exp e

pI Theor/Exp f

Expression level g A B C D

Fold change (P < 0.05) h RL Sk

8

Epidermis-specific secreted glycoprotein EP1-like Citrus sinensis

XP_006477736

155/1e-08

48.8/18

6.26/6.92

−3.81 np

10

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial-like Citrus sinensis

XP_006475040

67/4e-15

51.1/42

9.13/6.55

−2.11 2.17

13

epidermis-specific secreted glycoprotein EP1-like Citrus sinensis

XP_006477736

130/0.0

48.8/59

6.27/6.61

−1.82 1.50

15

miraculin-like protein 1 Citrus maxima

AEK31192

176/1e-20

18.9/15

8.18/5.80

2.32 1

16

Germin-like protein subfamily T member 2-like Citrus sinensis

XP_006477534

235/2e-169

25.9/24

5.74/5.35

−1.65 1.2

18

Germin-like protein subfamily T member 2-like Citrus sinensis

XP_006477534

235/2e-169

25.9/24

5.74/5.15

−1.57 -1.38

19

Germin-like protein subfamily T member 2-like Citrus sinensis

XP_006477534

235/2e-169

25.9/27

5.74/5.96

−1.39 2.27

20

Nucleoside diphosphate kinase Citrus sinensis

XP_006464834

60/2e-09

16.3/13

5.91/6.21

Np 1

21

2-phospho-D-glycerate hydrolase Citrus trifoliata

ADD12953

911/2e-18

47.77/39

5.42/5.57

−1.86 1.56

25

Annexin D2 Arabidopsis thaliana

NP_174810

160/6e-173

36.20/38

5.21/5.83

1.60 2.47

26

Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana

NP_175610

189/4e-14

48.20/50

6.08/5.79

−1.47 1

27

Lipase class 3 family protein Arabidopsis thaliana

NP_567515

150/0.0

59.06/59

9.33/5.86

−1.33 ∞

29

TIR-NBS-LRR type disease resistance protein Citrus trifoliata

AAN62351

93/2e-37

41.4/30

7.10/4.91

3.17 -1.62

34

Germin-like protein 3–3 like Citrus sinensis

XP_006477531

222/3e-31

43.3/24

5.73/6.07

1 -2.79

38

Germin-like protein 3–3 like Citrus sinensis

XP_006477531

222/3e-31

43.3/27

5.73/6.45

1 -3.45

40

Glyoxalase Theobroma cacao

XP_007026102

413/4e-144

27.06/33

6.52/5.64

1.73 -3.26

41

Mitochondrial malate dehydrogenase Citrus sinensis

AET22414

285/4e-98

30.89/34

5.2/6.10

−1.71 ∞

42

Chitinase Citrus sinensis

CAA93847

58/0.0045

32.45/35

5.06/4.80

3.44 np

46

Heat shock protein 70 Arabidopsis thaliana

CAA05547

77/0.0

71.4/46

5.14/6.32

1.64 1.76

48

2-phospho-D-glycerate hydrolase Citrus trifoliata

ADD12953

911/0.0

47.77/46

5.42/5.45

2.63 1

49

Methyl-CPG-binding domain 6 protein Arabidopsis thaliana

NP_200746

150/2e-43

24.44/49

9.03/6.0

1.35 2.07

51

Annexin 1 Theobroma cacao

NP_174810

109/0.0

35.8/48

6.34/5.72

2.16 np

54

2-phospho-D-glycerate hydrolase Citrus trifoliata

ADD12953

911/0.0

47.77/51

5.42/5.7

−1.94 -2.53

55

Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana

NP_175610

545/0.0

54.4/56

5.94/5.9

1.42 ∞

57

Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana

NP_175610

545/0.0

60.79/57

7.06/6.26

1.83 1.82

59

Annexin 1 Theobroma cacao

EOY16019

566/0.0

35.8/36.1

6.34/5.42

np 2.09

60

Annexin 1 Theobroma cacao

EOY16019

566/0.0

35.8/36.1

6.34/5.42

np 2.38

61

Lactoylglutathione lyase Citrus X paradisi

CAB09799

598/0.0

32.63/66

5.28/6.05

−1.81 1.66

63

Lactoylglutathione lyase S-transferase Ricinus communis

XP_002518470

52/8e-146

31.5/66

7.63/5.99

−1.90 1.65

70

Peroxidase Citrus maxima

ABG49115

517/0.0

37.88/44

4.52/6.10

−3.21 1

71

Histone ubiquitination proteins group Populus trichocarpa

XP_002302510

188/0.0

48.1/67

5.56/5.71

np −3.61

72

Acyl-CoA -N-acetyltransferase Arabidopsis thaliana

NP_196882

46.4/7e-29

20.39/22

7.8/5.48

np 1

75

5-formyltetrahydrofolate cyclo-ligase Arabidopsis thaliana

NP_565139

355/1e-119

39.55/39

9.41/6.63

np −2.20

79

2-phospho-D-glycerate hydrolase Citrus trifoliata

ADD12953

62.6/0.0

47.78/34

5.54/6.39

−2.68 np

93

mRNA-capping enzyme Arabidopsis thaliana

NP_974263

74.4/0.0

78.7/75.57

6.74/5.52

Np 1

94

Annexin D2 Citrus sinensis

CAB09799

116/4e-122

19.8/36

5.30/5.16

−1.79 np

100

ATP synthase beta subunit Citrus macroptera

ABM74441

69.4/3e-152

37.07/58

5.01/5.74

−2.68 -4.18

113

2-dehydro-3-deoxyphosphooctonate aldolase Medicago truncatula

ABN05924

427/2e-13

31.9/28

6.61/4.91

np ∞

116

Fructokinase Oryza sativa

A2WXV8

70/1e-34

30.3/87

5.50/6.19

np ∞

154

Heat shock protein-70 cognate protein Arabidopsis thaliana

NP_176036

73/0.0

71.4/65

5.10/5.27

∞ np

194

F-box family protein Vitis vinifera

XP_002279122

414/4e-139

47.2/55

9.4/6.39

−1.57 np

196

Fructokinase Citrus unshiu

AAS67872

219/2e-71

37.5/36

5.11/4.97

−2.54 1.64

202

Putative mitochondrial processing peptidase Arabidopsis thaliana

BAE98412

202/0.0

51.53/53

5.71/6.49

2.88 -1.95

205

Putative L-galactose dehydrogenase Citrus unshiu

ADV59927

294/1e-18

37.62/25

6.03/6.23

1.75 ∞

207

Putative mitochondrial processing peptidase Arabidopsis thaliana

BAE98412

480/0.0

51.53/55

5.71/6.33

1.56 1.47

  1. aSpot ID corresponding to the position in the 2D gel illustrated in Figure 1. bProtein accession number according to the NCBI database (http://www.ncbi.org). cBest matching protein identified by pBLAST analysis of the non-redundant (NCBInr) database. dMascot score P value of the homology between citrus proteins and orthologous, homologous, or paralogous proteins, as annotated in NCBInr. eTheoretical and experimental masses (KDa) of identified proteins. fTheoretical and experimental pIs of identified proteins. gExpression levels, presented as the % normalised volume, in the control and water deficit-stressed roots. Vertical bars indicate the mean ± SE. Rangpur lime: (A) control; and (B) water deficit. ‘Sunki Maravilha’: (C) control; and (D) water deficit. hFold change (water deficit-treated normalised volume/control normalised volume): bold = increased protein abundance; underlined = decreased protein abundance; italics = no significant difference; np = protein not found in gel; ∞ = present in one treatment in the genotype.