Figure 6From: Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus Pigment accumulation in the transgenic M. hupehensis callus with overexpressive CrtB gene. (A) Left image shows the phenotypes of the M. hupehensis calli cultured under visible light condition; middle image is the western blotting confirmation of CrtB protein, coomassie blue staining for loading control; right image displays the results of pigment (anthocyanins and carotenoids) levels analysis, columns and bars represent the means and ± SD, respectively (n = 3 replicate experiments); 35S:: CrtB represents the apple ECM. (B) Cytological investigation of M. hupehensis callus shows the amyloplasts in the wild-type cell, and the chromoplast-like structures in the ECM cell (arrows shown); s, starch granule; p, plastoglobule; m, mitochondrion. Bars represent 10 μm. (C) qRT-PCR analysis of anthocyanin related genes in the M. hupehensis calli. UFGT, uridine diphosphate (UDP)-glucose: flavonoid 3-O-glycosyltransferase; LDOX, leucoanthocyanidin dioxygenase; DFR1/2, dihydroflavonol 4-reductase 1/2; F3H, flavanone 3 β-hydroxylase; CHI, chalcone isomerase; CHS, chalcone synthase. Columns and bars represent the means and ± SD, respectively (n = 3 replicate experiments). 35S:: CrtB represents the light-cultured apple ECM; WT represents the light-cultured wild-type apple callus. (D) Phenotypes and pigment analyses under norflurazon treatment for 20 days. The medium contained 10 μM norflurazon. Columns and bars represent the means and ± SD, respectively (n = 3 replicate experiments). Transcript levels are expressed relative to the apple ECM (35S:: CrtB). * and ** indicate that the values are significantly different at the significance levels of P < 0.05 and P < 0.01, respectively.Back to article page