Figure 3

Evolutionary analysis of the newly-identified exons and their flanking exons. (A) Comparisons of evolutionary rates (dn, ds, and dn/ds) between the newly-identified exons and their flanking exons. Statistical significance was estimated by the paired two-tailed Wilcoxon signed rank-sum test. **P < 0.01 and ***P < 0.001. Error bars represent the standard errors of the means. (B) Proportions of newly-identified ASVs with and without significant differences in evolutionary rates between the novel exons and their flanking exons (P < 0.05 by the two-tailed Fisher’s exact test; Methods). Novel_dn and Novel_ds represent the dn and ds values of the novel exons; Flanking_dn and Flanking_ds represent the dn and ds values of their flanking exons, respectively.