Figure 4

Phylogenetic relationships among 37 species of the subfamily Thalictroideae. Tree was constructed using nucleotide sequence of five plastid genes/regions (rbcL, ndhF, ndhA intron, trnL intron, and trnL-F intergenic spacer). The gray ellipse on node indicates putative transfer of rpl32 to the nucleus and black dots indicate the complete loss of rpl32 from plastid. Black rectangle on node indicates an indel event that is shared by members of the T. coreaum clade. Species in bold are those surveyed for loss of rpl32. Bootstrap support values > 50% are shown at nodes. Tree in box shows the original ML tree, which is broken (-//-) in the tree on right to make it easier to visualize. The circumscription of the subfamily Thalictroideae follows Wang et al. [23].