Figure 3
Ppα -DOX expression in P. patens tissues. GUS staining of Ppα-DOX-GUS lines in; (A) border of a colony, (B) protonemal tissues at the border of a colony, (C) juvenile gametophore with GUS-stained young rhizoids (black arrow), GUS-stained long rhizoids with maximum staining in apical cells (arrowheads) and GUS-stained axillary hairs (red arrow), inset shows magnified axillary hairs, (D) adult gametophore with GUS-stained cells in the shoot apex (arrow) and parts of the cauloid, (E) GUS-stained dividing cells in detached leaf showing septa of cells that divided after leaf detachment (arrow), (F) protruded chloronemal cell facing the cut of the leaf, (G) protruded chloronemal cells of a detached leaf, (H) a closer view of G, (I) protoplast after regeneration for 4 days, (J) protoplasts after regeneration for 6 days, (K) protoplasts after regeneration for 7 days, and (L) young moss colony after protoplast regeneration for 11 days. Scale bars:20 μm in E-L; 100 μm in B; 0,5 mm C, D; 0,5 cm in A.