Figure 4

2-BP induced fragmentation and cross-linking of actin MF in root hairs. 4 DAG seedlings of Pro _35S :GFP-ABD2-GFP transgenic seedlings were treated with DMSO or with 10 μM 2-BP for 2–3 hr before imaging. 18 to 20 root hairs at different stages were examined and representative images are shown. Single section indicates one optical section taken at the mid-plane of a root hair. For each root hair shown, twenty 1 μm optical sections were superimposed to generate the projection of Z-stacks. Bars = 10 μm.