Figure 3From: The E3 ligase OsPUB15 interacts with the receptor-like kinase PID2 and regulates plant cell death and innate immunity The ARM repeat domain is required for OsPUB15 to be phosphorylated by PID2K. In vitro phosphorylation analysis of OsPUB15 (A), OsPUB15N (B), OsPUB15C-1 (C) or OsPUB15C-2 (D) by the kinase domain of PID2. The purified His fusion proteins were incubated with purified GST-PID2K in the presence of [γ-32P] ATP. GST-PID2K was used as a positive control. Samples were separated with SDS-PAGE followed by Coommassie Brilliant Blue staining (CBB staining) and Autoradiography (Autorad), respectively. Similar results were obtained from three independent experiments.Back to article page