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Figure 3 | BMC Plant Biology

Figure 3

From: The E3 ligase OsPUB15 interacts with the receptor-like kinase PID2 and regulates plant cell death and innate immunity

Figure 3

The ARM repeat domain is required for OsPUB15 to be phosphorylated by PID2K. In vitro phosphorylation analysis of OsPUB15 (A), OsPUB15N (B), OsPUB15C-1 (C) or OsPUB15C-2 (D) by the kinase domain of PID2. The purified His fusion proteins were incubated with purified GST-PID2K in the presence of [γ-32P] ATP. GST-PID2K was used as a positive control. Samples were separated with SDS-PAGE followed by Coommassie Brilliant Blue staining (CBB staining) and Autoradiography (Autorad), respectively. Similar results were obtained from three independent experiments.

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