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Figure 5 | BMC Plant Biology

Figure 5

From: DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

Figure 5

DAG2 expression is regulated by RGA and GAI. Relative expression level of: DAG2 in rga28 (A), and gai-t6 mutant seeds (B), and of RGA (C) or GAI (D) in dag2 mutant seeds, compared to wild-type seeds. Seeds were imbibed 12 hours in the dark (D), or under R light (R). Relative expression levels were normalized with that of PP2A (At1g13320) (A, B), or of UBQ10 (C, D), and are presented by the ratio of the corresponding wild-type mRNA level in D, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05).

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