Figure 2

Accumulation of N assimilatory enzymes in maize in response to inorganic N sources. 1D-WB performed against NR (A, C) and GS (B, D) in roots (A, B) and leaves (C, D) of maize plants grown without N sources for 10 days and then exposed for the last 30 h to absence of N (c), to 10 mM NO₃ ⁻ (n), to 10 mM NH₄ ⁺ (a) or to 5 mM NO₃ ⁻ + 5 mM NH₄ ⁺ (na). The analyses were conducted twice on two independent biological samples (n = 4) for each condition. B and D profiles were visualized by a global antibody for both GS1 and GS2 that recognized three different bands. The molecular masses of electrophoretic standards are reported on the left (kDa).