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Figure 1 | BMC Plant Biology

Figure 1

From: High-resolution confocal imaging of wall ingrowth deposition in plant transfer cells: Semi-quantitative analysis of phloem parenchyma transfer cell development in leaf minor veins of Arabidopsis

Figure 1

Confocal imaging of wall ingrowths in PP TCs of Arabidopsis leaf minor veins stained by the mPS-PI procedure. A. Single confocal section of a minor vein junction revealing polarized deposition of wall ingrowths (arrows) on the face of PP TCs (single asterisks) adjoining CCs. Polarized deposition of wall ingrowth material can be seen in other PP TCs, including the central PP TC (double asterisk) where ingrowth deposition is directed to opposite faces of the PP TC, each adjoining a different CC. The yellow dotted lines labelled y-z and x-z correspond to the projections shown in B and C, respectively. B. y-z projection of a z-stack of the image shown in A revealing finger-like projections (arrows) of wall ingrowth material extending from the face of two linearly-arranged PP TCs (asterisks) adjacent to neighboring SEs. C. x-z projection of a z-stack of the image shown in A revealing minor vein architecture in transverse section and the presence of highly-localized depositions of wall ingrowth material (arrowheads) adjacent to small SEs (asterisks) and larger CCs. D. Bright-field image of minor vein junction. The boxed area corresponds to the region shown in A and indicates the clarity of viewing vascular tissue when the abaxial epidermal layer is removed and the tissue is viewed from the abaxial surface of the leaf. BS, bundle sheath cell; CC, companion cell; SE, sieve element. Scale bar = 10 μm in A, B and C. Scale bar = 100 μm in D.

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