Fig. 6
From: Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae
DNA methylation and EMSA analysis of B. napus Monkey King. (a) and (b) DNA methylation detection results in the Monkey King sequence and its flanking sequences in the BnFLC.A10 promoter in Tapidor and Ningyou7. Each cell represents a cytosine. Blank cells denote no methylation. The higher the saturation is, the higher the DNA methylation level. (c) EMSA results in Tapidor. Thirteen probes (ES1-ES13) were derived from the Monkey King sequence. The binding ability of the probes to nuclear proteins from Tapidor leaves was analyzed by gel shift assays. Plus 50-fold (50) and without plus unlabeled probes (0) were used for the binding assays