Table 2 Details of 15 genes and the housekeeping gene Actin which were studied with qRT-PCR in seedling leaves harvested from the greenhouse trial in the 509 B. napus inbreds
Abb. a | Gene name | Amplicon size | Organism b | Reference | Start position | Primer sequence | No. of qRT-PCR |
|---|---|---|---|---|---|---|---|
of primer sequence | cycles | ||||||
CEL16 | Endo-1,4-beta-D-glucanase | 112 | B. napus | AJ242807.1 | 147 | 5‘-GGCTTCTGCATCCATTGTCT-3‘ | 45 |
(cellulase) | 258 | 5‘-TGCACTGTATCTGCCTCTCCT-3‘ | |||||
FBP | Fructose-1,6-bisphosphatase | 111 | B. napus mRNA | AF081796.1 | 81 | 5‘-GCCTCATCTCAGCCACAAAT-3‘ | 45 |
precursor | 191 | 5‘-AACCGCCATACACCTCACTC-3‘ | |||||
SPS | Sucrose-phosphate synthase | 106 | B. rapa subsp. | AY184484.1 | 194 | 5‘-CAGATGGGAACGAGGAACAT-3‘ | 45 |
pekinensis mRNA | 299 | 5‘-CTCGCAAGGGCAAGTATCAT-3‘ | |||||
RBC | Ribulose 1,5-bisphosphate carboxy- | 96 | B. napus mRNA | X07367.1 | 378 | 5‘-ACTACGATGGCCGTTACTGG-3‘ | 45 |
lase/oxygenase small subunit | 473 | 5‘-CCGTTTTGCACTCTTGGACT-3‘ | |||||
PK | Pyruvate kinase | 89 | B. napus mRNA | DQ539628.1 | 22 | 5‘-CCAAGGTTGTGGTGCTGAT-3‘ | 45 |
110 | 5‘-CTGATGCGGTGATAATGGAAT-3‘ | ||||||
PECT | Ethanolamine-phosphate | 192 | A. thaliana mRNA | NM_129424.4 | 483 | 5‘-CTGTGAAGTGGGTGGATGAA-3‘ | 45 |
cytidylyltransferase | 674 | 5‘-CAGTGCGCTTAATCTGCTTG-3‘ | |||||
APL | Glucose-1-phosphate adenylyl- | 195 | A. thaliana mRNA | NM_121927.3 | 581 | 5‘-CACTCAAACGCCAGGAGAAT-3‘ | 55 |
transferase large subunit 1 | 775 | 5‘-CTTATATCCGCGCCACTCTG-3‘ | |||||
AILP1 | Aluminum-induced protein | 123 | B. napus mRNA | JCVI_24 | 663 | 5‘-CTTGCTAAAAGGGGCTTGTG-3‘ | 45 |
785 | 5‘-GCAGGAATGGCAGTGATCTT-3‘ | ||||||
GER1 | Germin-like protein subfamily 3 | 189 | B. napus mRNA | JCVI_391 | 385 | 5‘-ATCACCGCTGGATTCATCTC-3‘ | 45 |
member 1 | 573 | 5‘-AGCAAATAGCGCAAAGTCAAG-3‘ | |||||
NOI | nitrate-induced domain protein | 198 | B. napus mRNA | JCVI_10152 | 249 | 5‘-CCAGCATCAGCAGAAGGTTT-3‘ | 45 |
446 | 5‘-TTGTGTCCGTCAAGAGTCCA-3‘ | ||||||
MyAP | myrosinase-associated protein | 131 | B. napus mRNA | JCVI_1353 | 34 | 5‘-AAAATGGCACCCAACTTCAG-3‘ | 45 |
164 | 5‘-TTGGAATCTCCGAATGTGAAC-3‘ | ||||||
GRF1 | Growth-regulating factor 1 | 101 | B. napus mRNA | JCVI_41561 | 121 | 5‘-CTTGGAGTTCGGTTTGAAGG-3‘ | 45 |
221 | 5‘-CCGGATCTTTCTTGCTTGTT-3‘ | ||||||
VPS2 | ESCRT c III complex | 123 | B. napus mRNA | JCVI_31511 | 148 | 5‘-TTTGCACCAGCAAGAGAGG-3‘ | 45 |
270 | 5‘-GGCTTGTTTCATGTGTGACG-3‘ | ||||||
UBP15 | Ubiquitin carboxyl-terminal | 117 | B. napus mRNA | JCVI_5013 | 676 | 5‘-TGAGAGGCAACTGGTTCAGA-3‘ | 45 |
hydrolase 15 | 792 | 5‘-TTAGAGGACGCGGATACGAT-3‘ | |||||
GF14 | G box factor 14-3-3 omega | 145 | B. napus mRNA | JCVI_22791 | 621 | 5‘-TTGCCCATTCGCTTTTATTC-3‘ | 45 |
protein | 765 | 5‘-AAGGTTCGATGGCAGACACT-3‘ | |||||
ACT | Actin | 104 | B. napus | GQ339782 | 156 | 5‘-TCAGGCCGTTCTTTCTCTTTAC-3‘ | 45 |
259 | 5‘-GAGCATAACCCTCGTAGATTGG-3‘ |