Linkage mapping, molecular cloning and functional analysis of soybean gene Fg3 encoding flavonol 3-O-glucoside/galactoside (1 → 2) glucosyltransferase

Table 1 PCR primers used in this study

Purpose	Target gene	Forward primer (5′-3′)	Reverse primer (5′-3′)
Cloning of cDNA for sequencing	GmF3G2″Gt	AATCACGTCCTTTGCACATA	CAAAACCATTGCCATTCATG
Sequencing of cDNA	GmF3G2″Gt	AGGCTGTGCACTATTGTACT	TCACAATGCTCACAGCTTTA
		CCATATATGGATTACATTGG	CACTTCCAAAGCAACAATAA
		TTCTTGCACACCCTTCTGTA	GACAGAGCTAGCAGTACAAT
Cloning of 5′ upstream region^a	GmF3G2″Gt		GTGTGTGTCTCTGGTGTAGTAATCATG
Cloning of 5′ upstream region^a	GmF3G2″Gt		CTCACACAAAACAGAGGTTCAATACGAGG
Sequencing of 5′ upstream region	GmF3G2″Gt	ACAACGGTCATATATGCATG	TGGCGGAGAGGAAGAAGATA
		ACAACGGTCATTTCTGCATG	CTCTCTGAAATGGATCCCAA
		GTCAACAATGAAGGACATGG	TAGAAATGCACGTAGTAGGT
			TCCACCACAATATGCTATGT
dCAPS analysis	GmF3G2″Gt	CCAAACTCACGAGGCTGGT	TCCTCCAAATCTAAAGTTGG
Cloning for functional analysis^b	GmF3G2″Gt	GCCGGAGCTCATGAAATCACGTCCTTTGC	GCCGCTCGAGTCAAATTCCCTCTACAATCTC
Real-time PCR	GmF3G2″Gt	AGAACCAAAGGATCTCCGTT	GTTTTTGCCAAGGAGATAAGT
Real-time PCR	actin	GTCCTTTCAGGAGGTACAACC	CCACATCTGCTGGAAGGTGC

^aGene-specific primers for genome walking.
^bRestriction sites incorporated in primers, SacI site in forward primer and XhoI site in reverse primer are italicized.

ISSN: 1471-2229