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Fig. 4 | BMC Plant Biology

Fig. 4

From: Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)

Fig. 4

Hierarchical clustering and quantitative analysis of HSFs and DREBs’ expression in response to DS, HS and HD. (a) Heat map showing the expression patterns of stress responsive HSFs. Two specific groups of HSFs exhibiting DS/HD or HS/HD up-regulated expression patterns including five and 24 HSF genes respectively, were identified. (b) Heat map showing the expression patterns of stress responsive DREBs. Two specific groups of DREBs exhibiting DS/HD or HS/HD up-regulated expression patterns including five and four DREB genes respectively, were identified. (c) Experimental validation of 10 randomly selected HSFs and DREBs by using quantitative RT-PCR. The expression patterns of two cluster1-HSFs, four cluster2-HSFs, two cluster1-DREBs and two cluster2-DREBs were validated after DS, HS and HD treatments at 1 h and 6 h, which exhibited similar expression patterns compared to the results revealed by RNA-Seq data

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