Fig. 5

Predicted transcriptional modules regulating wheat responses to DS, HS and HD. (a) GO terms (rounded rectangle) that are significantly overrepresented (p < 0.01, Fisher's exact test) within the DS/HD induced DREBs and HSFs co-expressed gene sets. Green rounded rectangle represents specific functional categories enriched in Cluster1-DREBs potentially regulated genes, red for Cluster1-HSFs and blue for both. A proportion of co-expressed transcription factors are also represented, arrows with solid lines indicate those TFs have been reported to be involved in drought stress responses, whereas dash lines represent TFs conferring tolerance to other abiotic stresses. (b) GO categories that are significantly overrepresented (p < 0.01, Fisher's exact test) within the HS/HD induced DREBs and HSFs co-expressed gene sets. Green rounded rectangle represents specific functional categories enriched in Cluster2-DREBs potentially regulated genes, red for Cluster2-HSFs and blue for both. A proportion of co-expressed transcription factors are also represented, arrows with solid lines indicate those TFs have been reported to be involved in heat stress responses, whereas dash lines represent TFs conferring tolerance to other abiotic stresses. (c) Comparison of GO categories enriched in two groups of predicted DREBs or HSFs target genes. Almost half of GO categories were shared by both groups. Gray rounded rectangle contains GO terms belonging to Group1 and black rounded rectangle contains GO terms belonging to Group2