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Fig. 6 | BMC Plant Biology

Fig. 6

From: Localization and expression of EDS5H a homologue of the SA transporter EDS5

Fig. 6

Characterization of the EDS5H-RNAi mutant and eds5-3/EDS5H-RNAi double mutant. a) Expression analysis of EDS5H and EDS5 in different genotypes. Plants were inoculated with 10 mM MgCl2 (M) or P. syringae pv. tomato DC3000. Samples were taken at 6, 12, 24, and 36 hpi. Northern blots were hybridized with gene-specific probes for EDS5H and EDS5. Ethidium bromide staining of the RNA gel (rRNA) was used as loading control. Note that some EDS5 expression can be detected as eds5-3 is not a transcriptional null mutant. The experiment was repeated 3 times with similar results. b) Accumulation of SA in different genotypes. Plants were inoculated with 10 mM MgCl2 (Mock) or P. syringae pv. tomato DC3000 carrying avrRpt2 (Avr). Samples were taken 12 and 24 hpi. Different letters above each bar represent statistically significant differences. The mean comparison of total SA was analyzed using Duncan’s multiple-range test (DMRT) with p-value ≤ 0.05. For each time point n = 4 (±SD), the experiment was repeated 3 times

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