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Fig. 4 | BMC Plant Biology

Fig. 4

From: The maize cytochrome P450 CYP79A61 produces phenylacetaldoxime and indole-3-acetaldoxime in heterologous systems and might contribute to plant defense and auxin formation

Fig. 4

Biochemical characterization of CYP79A61. Yeast microsomes containing the heterologously-expressed enzyme were prepared and incubated with the substrates L-phenylalanine and L-tryptophan. Time courses for the product formation in the presence of either 100 μM or 1 mM substrate are shown in a. The Michaelis-Menten kinetics for L-phenylalanine and L-tryptophan are given in b and the pH dependency of CYP79A61 product formation is illustrated in c. Products were detected using LC-MS/MS analysis with multiple reaction monitoring in the positive mode. Diagnostic reactions for each product: phenylacetaldoxime, m/z 136.0/119.0; indole-3-acetaldoxime, m/z 175.0/158.0

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