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Fig. 6 | BMC Plant Biology

Fig. 6

From: The maize cytochrome P450 CYP79A61 produces phenylacetaldoxime and indole-3-acetaldoxime in heterologous systems and might contribute to plant defense and auxin formation

Fig. 6

The response of maize leaves to simulated herbivory. CYP79A61 gene expression a, L-phenylalanine b and L-tryptophan c accumulation, (E/Z)-phenylacetaldoxime content d, and phenylacetic acid e and indole-3-acetic acid f levels were measured in undamaged leaves (ctr) and leaves subjected to simulated herbivory (herb). (E/Z)-phenylacetaldoxime, L-phenylalanine, L-tryptophan, and the auxins phenylacetic acid and indole-3-acetic acid were extracted with methanol and analyzed by LC-MS/MS. Gene expression was determined by qRT-PCR. Means and standard errors are shown (n = 5). Asterisks indicate statistical significance in Student’s t-test. Gene expression: p < 0.001; t = −4.99; L-phenylalanine: p < 0.001, t = 15.242; L-tryptophan: p < 0.001, t = 16.293; phenylacetaldoxime: p = < 0.001, t = 6.934; phenylacetic acid: p = < 0.001 , t = −18.259; indole-3-acetic acid: p = < 0.001, t = −5.644

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BMC Plant Biology

ISSN: 1471-2229

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