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Fig. 5 | BMC Plant Biology

Fig. 5

From: Tomato Sl3-MMP, a member of the Matrix metalloproteinase family, is required for disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

Fig. 5

Silencing of Sl3-MMP resulted in reduced resistance to B. cinerea. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-Sl-MMP or pTRV2-GUS and were inoculated at 4 weeks after VIGS infiltration by dropping spore suspension (1 × 105 spores/mL) on detached leaves or foliar spraying with spore suspension (2 × 105 spores/mL) onto leaves of whole plants. a and b Disease phenotype and lesion sizes in leaves of the pTRV2-Sl-MMPs- and pTRV2-GUS-infiltrated plants in detached leaf inoculation assays. Lesion sizes were measured at 3 days after inoculation on a minimum of 20 leaves in each experiment. c and d Disease phenotype on and fungal growth in the pTRV2-Sl3-MMP- and pTRV2-GUS-infiltrated plants in whole plant inoculation assays. Fungal growth in planta was estimated by analyzing the transcript levels of BcActin gene by qRT-PCR using SlActin as an internal control at the indicated time points after inoculation. Data presented in b and d are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level

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