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Fig. 8 | BMC Plant Biology

Fig. 8

From: Tomato Sl3-MMP, a member of the Matrix metalloproteinase family, is required for disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000

Fig. 8

Silencing of Sl3-MMP affected the expression of ROS generation- and scavenging-related genes and defense-mediated genes after infection of B. cinerea. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-Sl3-MMP or pTRV2-GUS and were inoculated with spore suspension of B. cinerea or with buffer as a mock-inoculation control at 4 weeks after VIGS infiltration. Leaves from six individual plants were collected at 24 h after inoculation and used for analysis of gene expression. a Expression of ROS generation- and scavenging-related genes. b Expression of defense-related genes. Relative expression levels were shown as folds of the actin transcript values. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level. MK, mock-inoculated control; Bc, B. cinerea-inoculated treatment

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