Fig. 1
Protein structure of LRX3, LRX4, and LRX5 and gene expression. a Leucine-rich repeat extensin (LRX) proteins consist of a signal peptide for protein export (black), followed by a variable domain, nine complete leucine-rich repeats (LRR, grey), a Cys-rich hinge region (dotted), and a C-terminal extensin domain (dark grey) that show the typical Ser-Hyp4 motifs of hydroxyproline-rich glycoproteins. Numbers indicate amino acid positions, arrowheads the positions corresponding to the T-DNA insertions in the identified mutants. b RT-PCR on total RNA extracted from wild-type and mutant seedlings with gene-specific primers for each of the three LRX genes and ACTIN2 as an internal control