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Fig. 4 | BMC Plant Biology

Fig. 4

From: Arabidopsis plants deficient in constitutive class profilins reveal independent and quantitative genetic effects

Fig. 4

qRT-PCR data and western blot analysis for double and triple mutant/ RNAi lines. Transcript expression ( a , b , & c ) a qRT-PCR data for T-DNA double mutants. Each graph shows the RQ of PRF1, PRF2, or PRF3 expression levels for each of the T-DNA double mutants. b qRT-PCR data for PRF1PRF2PRF3-RNAi lines (three lines shown). Each graph shows the RQ of PRF1, PRF2, or PRF3 expression levels for each of the PRF1PRF2PRF3-RNAi lines. c qRT-PCR data for PRF1PRF2-RNAi lines (three lines shown). Each graph shows the RQ of either PRF1 or PRF2 expression levels for each of the PRF1PRF2-RNAi lines. Error bars represent +/- 1 SD. Protein expression ( d & e ) d Western analysis of protein levels in profilin double mutants (all three combinations) using the PRF1 and PRF2 specific monoclonal antibody mAbPRF12a. e Western analysis of PRF1PRF2-RNAi and PRF1PRF2PRF3-RNAi lines using the PRF1 and PRF2 specific monoclonal antibody mAbPRF12a. Coomassie stained gels showing rubisco protein expression are located beneath each blot to show equal loading across lanes. All samples were taken from 4w old leaf tissue

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