Fig. 5
From: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp
Subcellular localization of five VpCDPKs. The selected CDPK genes were cloned from Chinese wild grape (Vitis pseudoreticulata) and used to construct CaMV35S::CDPKs–GFP vectors in which GFP was fused at the C terminus. The five VpCDPK-GFP fusion proteins (VpCDPK2-GFP, VpCDPK3-GFP, VpCDPK5-GFP, VpCDPK10-GFP, and VpCDPK11-GFP) as well as GFP as the control, were transiently expressed in Col-0 Arabidopsis protoplasts and observed by fluorescence microscopy. The merged pictures include the green fluorescence channel (first panels) and the chloroplast autofluorescence channel (second panels). The corresponding bright field images are shown on the right. Bar = 5 μm