Fig. 5

In situ hybridization analysis of monocot rpk1-7 seedlings. Shown are (serial) sections of torpedo embryos (dicot and monocot embryos indicated). a–e6 In situ hybridization with the STM probe. f-g4 In situ hybridization with the ENP probe for comparison. a-c8 and f-g4 show longitudinal sections and d1-6; e1-6 show cross-sections respectively. Brackets in a and d2-5, e2-5 indicate the distance of 15-20 μm (cross sections have 3,5 μm thickness). The stippled line in b2 and b3 indicates the extension of the STM signal along the SAM region and the hypocotyl. Arrows point to the localized SAM signals of STM and (late) ENP respectively. Arrowheads indicate the additional ENP signal in the cotyledon epidermis. Scale bars: 20 μm