Fig. 5

Conservation of rRNA methylation in Kingdom Plantae. a Concentric circles from outer to inner represent Arabidopsis thaliana (At), Brassica rapa (Br), Triticum durum (Td), Nannochloropsis oculata (No), Caulerpa taxifolia (Ct) and Ginkgo biloba (Gb) rRNAs, respectively. The circles are split into three sections for nuclear, chloroplast and mitochondrial encoded rRNAs. In each circle, individual rRNA sequences are represented as thick grey bars. The nucleotide positions for each rRNA species are based on alignment with the corresponding Arabidopsis consensus sequences. Cytosines that are methylated in at least one of the 6 species analysed are shown as a color-coded percentage methylation bar. In the percentage methylation scheme used, Green = lowly methylated (0–40 %), red = highly methylated (80–100 %). Absence of a methylation bar indicates that the corresponding position in the rRNA does not contain a cytosine in that species. Open triangle indicates where consecutive cytosines are methylated in Triticum durum. The black methylation bar at position 1703 in Nannochloropsis oculata SSU 18S rRNA shows where the sequence could not be aligned to the Arabidopsis reference sequence (At 3 replicates and other species 1 replicate)