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Fig. 1 | BMC Plant Biology

Fig. 1

From: The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana

Fig. 1

Identification of a suppressor of hsr8-1 sugar-hypersensitive hypocotyl elongation in the dark. a Image of sugar hypersensitive hypocotyl elongation of Col, hsr8-1 and hsr8-1soh715 grown on 1 % glucose on vertical plates in the dark. b Quantitative measurements of hypocotyl lengths of Col, hsr8-1 and hsr8-1soh715. Seedlings were grown vertically in the dark for 14 days on MS medium with 1 % Glucose. Errors bars represent SD (n > 30). ***, p < 0.001 comparing Col to hsr8-1 and hsr8-1 to soh715 hsr8-1 (Student’s t- test). Data shown is representative of three independent experiments. c Quantitative Real-time PCR analysis of β-Amylase mRNA levels in Col, hsr8-1 and the hsr8-1soh715 repressor in response to glucose. Seedlings were grown on MS medium supplemented with 0.5 % glucose in constant light. After 7 days, seedlings were transferred 24 h in a MS glucose-free liquid medium and then treated for 6 h with 3 % MS medium containing 3 % glucose. Errors bars represent SD from three biological replicates. Data shown is representative of three independent experiments. **, p < 0.01 comparing Col to hsr8-1; ***, p < 0.001 comparing hsr8-1 to soh715 hsr8-1 (Student’s t- test). Relative transcript levels (RTL) were calculated using transcript levels of the reference gene TUB6 (At5g12250). d Anthocyanin accumulation in response to glucose in Col, hsr8-1 and hsr8-1soh715. Seedlings were grown in continuous light for 7 days on MS medium containing 1 % glucose (solid bars) or 3 % glucose (dashed bars). Errors bars represent SD from three biological replicates. **, p < 0.01 comparing Col to hsr8-1; ***, p < 0.001 comparing hsr8-1 to soh715 hsr8-1 (Student’s t- test). Data shown is representative of two independent experiments

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