Fig. 1
From: Metabolome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants
Metabolomics workflow used in this study. Five different analytical platforms (purple boxes) were employed to analyze plant tissues derived from 20 BIA-accumulating species. Metabolite classes measured using targeted methods are indicated. Identification and quantification were achieved using software packages (NMR, LC/DI-MS/MS) or manually (HPLC-UV, UPLC-FTMS). Untargeted UPLC-FTMS (+)-mode data was mined for exact masses corresponding to alkaloids. Targeted analysis using triple quadrupole LC-MS/MS was performed to acquire additional identifying information. Abbreviations: BIA, benzylisoquinoline alkaloid; NMR, nuclear magnetic resonance; LC, liquid chromatography; DFI, direct flow injection; MS, mass spectrometry; MS/MS, tandem mass spectrometry; HPLC, high-performance liquid chromatography; UV, ultraviolet light absorption spectroscopy; UPLC, ultra performance liquid chromatography; FTMS, Fourier transform mass spectrometry; ESI, electrospray ionization