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Fig. 10 | BMC Plant Biology

Fig. 10

From: Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium

Fig. 10

In vitro assays indicate that DnSIZ1 is a functional SUMO E3 ligase. a pCDFDuet-1-Flag-DnSIZ1 was expressed in Escherichia coli and then tested for SUMOylation activity in the presence of E1 (His-AtSAE1a-AtSAE2), E2 (His-AtSCE1) and Myc-SUMO1. b pCDFDuet-1-Flag-DnSIZ1 and pCDFDuet-1-Flag-DnSIZ1C380A were expressed in Escherichia coli and then tested for SUMOylation activity. Immunoblots generated from these samples were probed with anti-Flag antibodies

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