Fig. 10From: Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium In vitro assays indicate that DnSIZ1 is a functional SUMO E3 ligase. a pCDFDuet-1-Flag-DnSIZ1 was expressed in Escherichia coli and then tested for SUMOylation activity in the presence of E1 (His-AtSAE1a-AtSAE2), E2 (His-AtSCE1) and Myc-SUMO1. b pCDFDuet-1-Flag-DnSIZ1 and pCDFDuet-1-Flag-DnSIZ1C380A were expressed in Escherichia coli and then tested for SUMOylation activity. Immunoblots generated from these samples were probed with anti-Flag antibodiesBack to article page