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Fig. 7 | BMC Plant Biology

Fig. 7

From: Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium

Fig. 7

Inhibition of siz1-2 ABA-hypersensitivity by overexpression of DnSIZ1. Seeds of wild-type (col-0), siz1-2 (atsiz1-2), and each of the transgenic lines (HB4, HB17) were sown on MS medium without or supplemented with ABA. a Cotyledon expansion of transgenic plants in the presence of ABA. Stratified seeds of Col-0, siz1-2 (atsiz1-2), and each of the transgenic lines (HB4, HB17) were spread in the illustrated pattern on MS medium containing 0 and 0.2 μM ABA and maintained under a 16 h-light/8 h-dark conditions at 22 °C. b Germination rates of seedlings 48 h after stratification. c Cotyledon expansion of 5 day-old seedling in the presence or absence of 0.2 μM ABA. The data represent the averages of three independent experiments. The percentages (±SE) of seedlings with green cotyledon in each genotype are shown. Significant differences from atsiz1-2 (asterisks) at P < 0.05 are indicated

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