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Fig. 8 | BMC Plant Biology

Fig. 8

From: Tomato histone H2B monoubiquitination enzymes SlHUB1 and SlHUB2 contribute to disease resistance against Botrytis cinerea through modulating the balance between SA- and JA/ET-mediated signaling pathways

Fig. 8

Silencing of SlHUB1 and SlHUB2 resulted in accumulation of ROS and affected the expression of ROS generation- and scavenging-related genes after infection with B. cinerea. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlHUB1, pTRV2-SlHUB2 or pTRV2-GUS constructs and were inoculated by spraying with spore suspension of B. cinerea or with buffer as mock-inoculation control at 4 weeks after VIGS infiltration. At least 6 leaves from 6 individual plants were collected at 0 (as controls) and 24 h after inoculation. a Accumulation superoxide anion. b Accumulation of H2O2. Representative NBT- or DAB-stained leaves are shown and similar results were obtained from repeated experiments. c Expression of ROS generation- and scavenging-related genes before and after infection with B. cinerea. Relative expression levels were shown as folds of the actin transcript values. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level

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