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Fig. 3 | BMC Plant Biology

Fig. 3

From: In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca2+-effects

Fig. 3

Ca2+-impact on ATP and ADP transport of full-length and N-terminally truncated AtAPC2. Transport via recombinant AtAPC2 (a) and via the mutated version lacking its N-terminal domain (b). Import of [α32P]-ATP into Pi loaded proteoliposomes (black squares) and of [α32P]-ADP into ATP loaded vesicles (gray circles) was allowed for 5 min. Transport without CaCl2 was set to 100 % and transport in presence of rising concentrations of externally added CaCl2 (0 - 500 μM) was calculated accordingly. Data represent net values of ATP/Pi and ADP/ATP uptake minus the respective control (non-loaded vesicles) of three independent replicates. Standard errors are given

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