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Table 3 Effects of 200 μM calcium on KM-Values of adenine nucleotide transport

From: In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca2+-effects

Exchange

AtAPC1

AtAPC2

AtAPC3

 

KM

Vmax

KM

Vmax

KM

Vmax

ATP/Pi

68 (6)

201(±14)

95 (±12)

212 (±24)

113 (±17)

282 (±20)

ATP/Pi + Ca2+

61 (±6)

398 (±30)

59 (±6)

523 (±48)

58 (±8)

646 (±88)

ADP/ATP

180 (±15)

2078 (±315)

374 (±40)

778 (±82)

72 (±5)

770 (±90)

ADP/ATP + Ca2+

178 (±15)

2455 (±357)

508 (±94)

1169 (±126)

140 (±12)

1025 (±140)

  1. Transport was performed with rising ATP or ADP concentrations and allowed for 2.5 min. KM- values are given in μM and Vmax in nmol mg protein-1 h-1. Data represent the mean of at least three independent experiments. Standard errors are given in brackets