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Fig. 10 | BMC Plant Biology

Fig. 10

From: Two homolog wheat Glycogen Synthase Kinase 3/SHAGGY - like kinases are involved in brassinosteroid signaling

Fig. 10

Propiconazole treatment of wheat embryos. Embryos were isolated out of the kernel at an embryo-proper diameter of 100 to 240 μm, most of them having a diameter of 120 to180 μm. The phenotypes of the in vitro grown embryos were observed at 5 to 28 days of culture and grouped into classes. Photographs of representative phenotypes were added directly to the table (a-c, 3photograph not shown). Reported numbers in (d) are percentages of defined phenotypes per growing embryos. In 5 independent experiments, 42 growing embryos were observed in control conditions (0.1 % DMSO), 61 in the case of 5 μM PCZ, 52 for 10 μM PCZ. 1days of culture. 2fewer control embryos isolated at sizes of 120–150 μm were developing in vitro into normal wild type embryos due to a higher amount of abnormal, undifferentiated or early arrested embryos. Cr: coleoptilar ring; RG: Radial Growth (BS: Ball-Shaped or PS: Pear-Shaped); sc or scut: scutellum; sm: shoot meristem; sp: suspensor; wt: embryo having a wild type phenotype. The percentage of indifferentiated, abnormal or early arrested embryos for a given concentration and a given embryo size range is obtained as in Fig. 9

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