Fig. 11

Bikinin treatment of wheat embryos. Embryos were isolated out of the kernel at sizes ranging from 100 to 240 μm in diameter, most of them having a diameter of 120 to 180 μm. The phenotypes of the in vitro grown embryos were observed between 4 and 13 days of culture. Photographs of representative phenotypes were added directly to the figure (a-e, ²photograph shown in additional file 6). Reported numbers in (f) are percentages of defined phenotypes per growing embryos. In three independent experiments, a total of 29, 20, 33 37, 31 and 32 growing embryos were observed respectively for the control, 2, 5, 10, 20 and 30 μM Bikinin. ¹ days of culture. apex: differentiation of a leaf primordium or/and a coleoptilar ring (both in most cases abnormal) in addition to a shoot meristem; cr: coleotilar ring; RG: Radial Growth (BS: Ball-Shaped or PS: Pear-Shaped); sc: scutellum; sm: shoot meristem; sp: suspensor; wt: embryo having a wild type phenotype. The percentage of indifferentiated, abnormal or early arrested embryos for a given concentration and a given embryo size range is obtained as in Fig. 9