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Fig. 5 | BMC Plant Biology

Fig. 5

From: Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge

Fig. 5

Heterologous expression of OoDAD1 and Western analysis. a Coomassie blue stained sodium dodecyl sulphate polyacrylamide gel showing over-expression of recombinant OoDAD1 in BL21 (DE3) pLysE Escherichia coli-based expression system using pET 28a expression vector. Lanes: 1, lysate of induced, transformed cells with OoDAD1; 2, supernatent of induced, transformed cells with OoDAD1; 3, pellet of induced, transformed cells with OoDAD1. M, protein ladder. b Western analysis of polyacrylamide gel shown in (a) and electrotransferred to a nitrocellulose membrane and probed with anti-His tag antibodies (see Experimental Procedures). Lane designation is same as in (a). Arrows indicate the location of the His-tagged OoDAD1. Numbers on the left represent molecular weights in kilodaltons (kDa)

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