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Table 1 List of primers used for cloning and quantitative Real Time assays of OoDAD1. The ‘Prot’ primers were used for cloning OoDAD1 in pET 28a expression vector

From: Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge

S.No

Name

Primer sequence (5′➔ 3′)

Annealing Temp. (°C)

Amplicon size (bp)

1

OoDAD-F

ATGACGAATCTAACTACAGTTGTTC

55

339a

2

OoDAD-R

CTAACCGATGAAATTGAATACG

 

424b

3

RTDAD-F

CATTGTGCTTACCGGTGTCATT

60

177

4

RTDAD-R

CGTTCCGGTGAGATTCCAAT

  

5

RTActin-F

TGAGACACCATCACCGGAATC

60

149

6

RTActin-R

ATCCAAAGGCCAATCGTGAA

  

7

ProtDAD-F

GAGACATATGACGAATCTAACTACAGTTGTTC

55

339

8

ProtDAD-R

GAGACTCGAGCTAACCGATGAAATTGAATACG

  
  1. aAmplicon size when cDNA was used as template
  2. bAmplicon size when genomic DNA was used as template