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Fig. 4 | BMC Plant Biology

Fig. 4

From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)

Fig. 4

In situ hybridization of RhAG mRNA accumulation in rose flower buds. RhAG was detected using a DIG-labeled probe in flower buds of rose plants grown under normal temperature (25/15 °C) (a-f) or low temperature (15/5 °C) (g-l) conditions. a and g, stage 1 floral buds; b and h, stage 2 floral buds; c and i, stage 3 floral buds; d and j, stage 4 floral buds. A sense probe was used for stage 3 (e and k) and stage 4 (f and l) buds as a negative control. SE, sepal; PE, petal; ST, stamen; CA, carpel. Arrows show the boundary between petal and stamen. Scale bars = 100 μm (a, b, g and h) or 200 μm (c-f; i-l)

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