Fig. 4From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida) In situ hybridization of RhAG mRNA accumulation in rose flower buds. RhAG was detected using a DIG-labeled probe in flower buds of rose plants grown under normal temperature (25/15 °C) (a-f) or low temperature (15/5 °C) (g-l) conditions. a and g, stage 1 floral buds; b and h, stage 2 floral buds; c and i, stage 3 floral buds; d and j, stage 4 floral buds. A sense probe was used for stage 3 (e and k) and stage 4 (f and l) buds as a negative control. SE, sepal; PE, petal; ST, stamen; CA, carpel. Arrows show the boundary between petal and stamen. Scale bars = 100 μm (a, b, g and h) or 200 μm (c-f; i-l)Back to article page