Fig. 5From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)Effects of low temperature on DNA methylation of the RhAG gene. The effects of low temperature (15/5 °C) on cytosine DNA methylation in the RhAG gene (a) were determined by McrBC digestion (b) and HaeIII-mediated Chop-PCR assays (c). a Schematic structure of the RhAG gene. The primers used for the Chop-PCR assay are indicated as F1 + R1, F2 + R2, and F3 + R3. b McrBC digestion assay. Genomic DNA was digested with McrBC for 3 h and amplified by PCR. c HaeIII-mediated Chop-PCR assay. Linearized genomic DNA was digested with HaeIII for 3 h and amplified by PCR. In each assay, undigested genomic DNA was used as a controlBack to article page