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Fig. 5 | BMC Plant Biology

Fig. 5

From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)

Fig. 5

Effects of low temperature on DNA methylation of the RhAG gene. The effects of low temperature (15/5 °C) on cytosine DNA methylation in the RhAG gene (a) were determined by McrBC digestion (b) and HaeIII-mediated Chop-PCR assays (c). a Schematic structure of the RhAG gene. The primers used for the Chop-PCR assay are indicated as F1 + R1, F2 + R2, and F3 + R3. b McrBC digestion assay. Genomic DNA was digested with McrBC for 3 h and amplified by PCR. c HaeIII-mediated Chop-PCR assay. Linearized genomic DNA was digested with HaeIII for 3 h and amplified by PCR. In each assay, undigested genomic DNA was used as a control

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