Fig. 5
From: Genetics and physiology of cell wall polysaccharides in the model C4 grass, Setaria viridis spp
Micrographs of leaf transverse sections. a Bright-field light micrograph of a toluidine blue-stained survey section. b-f Fluorescence light micrographs using b an absent of primary and secondary antibodies as negative control, c-f an antibody conjugated to Alexafluor 488. (1,3;1,4)-β-Glucan is indicated by green fluorescence. c Young leaf blade. d Old leaf blade. e Young leaf across midrib. f Old leaf across midrib. E, epidermis; BS, bundle sheath; M, mesophyll; Xy, xylem; Ph, phloem; SF, sclerenchyma fibre; GC, guard cells; VB, vascular bundle; BfC, bulliform cells. Magnifications: a, e 200 x; b–d, f 400x. Bars: a, e 100 μm, b–d, f 50 μm. Exposure time: b–f 1.6 s