Fig. 9
From: Genetics and physiology of cell wall polysaccharides in the model C4 grass, Setaria viridis spp
Micrographs of developing grain transverse sections. a-f Combination fluorescence light micrographs showing overlaid signals from both an antibody conjugated to Alexafluor 488 and Calcofluor White MR2. Fluorescence light micrographs using a a primary antibody only as negative control, g-l an antibody conjugated to Alexafluor 488 where (1,3;1,4)-β-glucans are indicated by green fluorescence. DPc, developing pericarp; Em, embryo; Sct, scutellum; Ct, coleoptile; Cr, coleorhiza; Es, endosperm. Magnification: a-c, g-i 5 x; d-f, j, k 100x; (l) 40x. Scale Bars: a-c, g-i 200 μm; d-f, j, k 100 μm; 20 μm. Exposure time: a-c, g-i Alexafluor 488 = 860.4 ms, Calcofluor White MR2 = 700 ms; d-f, j, k Alexafluor 488 = 550, Calcofluor White MR2 = 260 ms; l Alexafluor 488 = 100 ms, Calcofluor White MR2 = 600 ms