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Fig. 2 | BMC Plant Biology

Fig. 2

From: Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

Fig. 2

Western blot analysis of curcin proteins in transgenic jatropha plants. a Detection of C1 proteins in the endosperm of transgenic T1 seeds by western blot analysis. T0-1/T1-1, T0-29/T1-1, T0-35/T1-1, T0-40A/T1-5 and T0-48/T1-19 are transgenic T1 seeds carrying RNAi cassettes derived from the respective T0 plants. b Detection of C2A proteins in young leaves of T0 plants by western blot analysis. Proteins isolated from the endosperm of mature jatropha seeds (a) or young leaves (b) were separated by 8 % SDS-PAGE. Curcin proteins were detected by anti-C1 antibodies. Proteins stained with Coomassie brilliant blue in duplicate SDS-PAGE gels served as protein loading controls. Arrows indicate the positions of C1 (a) and C2A (b), respectively. kDa, kilodalton; MD44, non-transgenic control

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BMC Plant Biology

ISSN: 1471-2229

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