Fig. 6From: Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencingNorthern blot analysis of C1 gene transcripts in the endosperm of T3 seeds of L1 and L35. Total RNA was isolated from 6-week-old immature seeds of MD44 and T3 progeny derived from the T2 individuals of L1 (T0-1/T1-1/T2-2 and T0-1/T1-1/T2-9) and L35 (T0-35/T1-1/T2-1 and T0-35/T1-1/T2-2). rRNAs on methylene blue-stained membranes are shown as a loading controlBack to article page