Skip to main content

Table 2 Summary of PCR analysis for T0 transgenic plantsa

From: Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

Name Gus linker F1-R2 Hpt F1-R1 F2-R2
MD44
T0-1 + + + +
T0-20A + + + + +
T0-25A + +
T0-29 + +
T0-30 +
T0-33 + + + + +
T0-34 +
T0-35 + + + + +
T0-36 + + +
T0-40A + + +
T0-40B + +
T0-48 + + + + +
  1. aDNA primer pairs for PCR amplification are as follows: Gus linker, Gus-L-F and Gus-L-R; F1-R2, F1 and R2; Hpt, Hpt-F1 and Hpt-R1; F1-R1, F1 and R1; F2-R2, F2 and R2. The DNA sequences of the primers are listed in Table 1