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Fig. 8 | BMC Plant Biology

Fig. 8

From: Oligogalacturonic acids promote tomato fruit ripening through the regulation of 1-aminocyclopropane-1-carboxylic acid synthesis at the transcriptional and post-translational levels

Fig. 8

Time-course analysis of phosphorylated LeACS2 treated with or without OGs. Proteins were extracted from samples the same for gene expression detection. Total 25 μg protein were loaded on each line to separate through 8 % SDS-PAGE gel, anti-phosphorylated-LeACS2 was incubated with membrane overnight at 4 °C. Ponceaux dyeing was used to verify the protein amount. The relative intensities of phosphorylated-LeACS2 were quantified with Adobe Photoshop CC. We used the second hour protein band intensity of the control group as standard. The experiment was repeated in triplicate. A representative gel is shown. Significant differences of four groups at each time point were indicated by different letters (Tukey’s HSD, P < 0.05)

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BMC Plant Biology

ISSN: 1471-2229

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