Fig. 1From: Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soilDe novo transcriptome assembly and analysis pipeline. Quality control - Paired-ends reads are filtered to remove poor quality reads and nucleotides. De novo assembly - Transcriptome is assembled de novo using Trinity. Expression analysis - Mean abundance of all Trinity genes by treatment. Region highlighted in red represents treatment asymmetry in fold change (FC) distribution. Unconstrained annotation - Gene annotation pipeline (see Fig. 2)Back to article page