Fig. 2From: Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soilSchematic approach; unconstrained and informed metatranscriptomic annotation. An initial unconstrained annotation is aimed at retention of metaorganismal data, allowing the potential for discoveries of system biology. Once informed, selection of annotation from very similar competing blast hits can be given an order of priority, as performed here: <10Â % difference in bitscore and protein coverage: i) S. purperea, ii) T. urticae, iii) SwissProt, iv) Trembl, v) NCBI nr. DB databaseBack to article page