Fig. 2

Proline-deficient mutants have meristems smaller than wild types. a-f Root meristems from wild type (a, c, e) and p5cs1 p5cs2/P5CS2 (b, d, f), at 3 (a, b), 5 (c, d) and 7 dag (e, f). Bottom black arrowheads indicate the QC, top black arrowheads indicate the cortex TZ. g Root meristem from p5cs1 p5cs2/P5CS2 treated, at 7 dag, with 10 μM exogenous proline. h-i Wild-type root, at 10 dag, treated with 10 μM exogenous proline (i) compared with an untreated control (h). Bars = 20 μm (a-i). j Root meristem cell number of plants described in (a) to (i) plotted over time from 1 to 10 dag. The data are the means ± SE of at least 3 independent experiments. A minimum of 50 roots per line was analyzed at each time point