Fig. 5

Proline affects the expression of CYCB1;1 in the root meristematic zone. a-p CYCB1::GUS expression, from 1 to 7 dag, in roots from CYCB1::GUS (a, e, i, m), p5cs1 p5cs2/P5CS2, CYCB1::GUS (b, f, j, n), proline-treated CYCB1::GUS (c, g, k, q) and proline-treated p5cs1 p5cs2/P5CS2, CYCB1::GUS (d, h, l, p). Bottom and top arrowheads show meristem size indicating the QC and, respectively, the TZ. Bars = 50 μm (a-d), 20 μm (e-p). q qRT-PCR of CYCB1;1, at 3 and 5 dag, in root meristems of wild type (dark grey bar), p5cs1 p5cs2/P5CS2 (light grey bar), and proline-treated p5cs1 p5cs2/P5CS2 (grey bar), showing, at 3 dag, a strong downregulation of CYB1;1 expression in p5cs1 p5cs2/P5CS2 roots. The meristem-specific gene RCH1 was used as reference control to normalize the qRT-PCR