Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Changes in hormone flux and signaling in white spruce (Picea glauca) seeds during the transition from dormancy to germination in response to temperature cues

Fig. 3

Changes in GAs and GA signalling components during the transition from dormancy to germination of white spruce seeds. a Profiles of the GA precursor GA53 and the metabolite GA34 as determined by UPLC/ESI-MS/MS during moist-chilling at 3 °C (0, 10, and 21 days), and during germination (6, 24 and 80 h) and seedling growth (9 d). Each data point is the average of two biological replicates. No active GAs were detected in our analysis. b Schematic characterization of key genes and their interplays in GA signaling cascades. Connections represent positive (arrow) and negative (block) regulation. c Transcript levels of GA metabolism genes and selected downstream targets during moist-chilling (0, 10, 21 d), germination (6, 24, 80 h) and seedling growth (9 d) (black bars). Also shown are previously chilled seeds placed in two control treatments - 6 h germination conditions under darkness at either 30 °C (dark grey bar) or 20 °C (light grey bar). Each data point is the average of three biological replicates. Bars indicate the SEM. Note: see Fig. 2 note for asterisks. d Ent-pgKS protein levels during moist-chilling, germination, and growth of white spruce seeds. Immunoblots show 30 μg of total protein extract per lane. Blots were probed with anti-KS antibody and anti-tubulin as a loading control

Back to article page